Codistirf detects diffraction limited object and measure the distance to the closest point of the membrane in multichannel imaging [1]. Codistirf is a high-troughput capable library on top of original contributions by Francois Aguet [2].
- Multiple channel support (tested with four channels)
- GraphPrysm export
- Automatic diffraction limited object and membrane detection
- GUI for membrane correction
- Support parallel batch processing for high-throughput microscopy (~1Gb processed per second measured on Intel(R) Xeon(R) E5-2680 v3 with 256Gb of RAM)
See script runFixedDualChannelAnalysis.m
Lakoduk, Ashley M., Philippe Roudot, Marcel Mettlen, Heather M. Grossman, Ping-Hung Chen, and Sandra L. Schmid. “Mutant P53 Triggers a Dynamin-1/APPL1 Endosome Feedback Loop That Regulates Β1 Integrin Recycling and Migration,” September 5, 2018. https://doi.org/10.1101/408815.
Reis, Carlos R, Ping-Hung Chen, Saipraveen Srinivasan, François Aguet, Marcel Mettlen, and Sandra L Schmid. “Crosstalk between Akt/GSK3β Signaling and Dynamin-1 Regulates Clathrin-Mediated Endocytosis.” The EMBO Journal 34, no. 16 (August 13, 2015): 2132–46. https://doi.org/10.15252/embj.201591518.
